abstract：:A high-performance liquid chromatographic method with electrochemical detection is described for the determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in brain. Results of studies on sample work-up methods and on the use of various internal standards are reported. The reproducibility ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Duchateau A,Martens H,Teuchy H,Schoups A,Claeys M

更新日期：1988-01-01 00:00:00

abstract：:Comparison is made between conventional liquid-liquid extraction and solid-phase extraction techniques using Extrelut-1, Extrelut-3 and C18-RP cartridges respectively for the screening of several beta-blockers in urine. Generally, using GC with nitrogen specific detection as the screening technique, liquid-liquid extr...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Delbeke FT,Debackere M,Desmet N,Maertens F

更新日期：1988-01-01 00:00:00

abstract：:The conditions of least squares deconvolution by linear combination have been investigated in three-component systems. The significant effects of the main factors, the lack of significant interactions and the linear relationship of absorbance with the concentration of each component were established by using a 2(3) fa...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Géher J,Szabó E

更新日期：1988-01-01 00:00:00

abstract：:A variety of analytical procedures have been described for the determination of cisplatin and its analogues in biological fluids (plasma, plasma ultrafiltrate and urine), as well as in solid tissues. This paper attempts to review those methods which have been most commonly used in practice. These analytical methods ma...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Riley CM

更新日期：1988-01-01 00:00:00

abstract：:To maximize the benefits of a LIMS, the system must be integrated with the analytical instrumentation in the laboratory. This provides on-line data capture or transfer of results for matching with the corresponding sample records held within the database, which reduces transcription error checking and ensures data int...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： McDowall RD

更新日期：1988-01-01 00:00:00

abstract：:Particle counting immunoassay (PACIA) is applied to the determination of proteins at trace levels in normal and pathological cerebrospinal fluid (CSF) samples. Performance characteristics for the assays are given and the results for immunoglobulins (Igs) expressed as Ig indices. Ig profiles characteristic of various n...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Sindic CJ

更新日期：1987-01-01 00:00:00

abstract：:The 60 MHz (1)H NMR spectra of racemic thiopental, 1, have been studied with the achiral shift reagent, tris(6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octanedionato) europium(III), 2, and the chiral tris[3-(trifluoromethylhydroxymethylene)-d-camphorato] europium(III), 3, and tris[3-(heptafluoropropylhydroxymethylene)...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Eberhart ST,Hatzis A,Jimenez J,Rothchild R,Simons P

更新日期：1987-01-01 00:00:00

abstract：:The current position with respect to the expression of biological potency and chemical purity of antibiotics is reviewed. In particular, attention is drawn to the policies adopted in the USA which have been a source of confusion regarding the expression of biological potency. It is suggested that the opportunity provi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Thomas AH

更新日期：1987-01-01 00:00:00

abstract：:A stationary phase has been designed and synthesized in which theophylline residues are covalently bonded to a silica support through an eight carbon hydrocarbon linkage. The phase offers improved resolution in the separation of aromatic carboxylic acids over that available with conventional reversed phase supports. T...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Apfel M,Sternson LA,Repta AJ,Mills D

更新日期：1987-01-01 00:00:00

abstract：:A new analytical method for chloramphenicol and its derivatives, chloramphenicol succinate sodium and D-(-)-threo-2-amino-L-[p-nitrophenyl]-1,3-propanediol (chloramphenicol base) in the pure state or in pharmaceutical preparations, is described. The method is based on measuring the blue colour produced by the interact...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Morelli B

更新日期：1987-01-01 00:00:00

abstract：:The spectral, solubility and related physicochemical characteristics of the ovarian hormone are presented. A review of its crystal properties indicates that the common crystalline form of the steroid is the hemihydrate. ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Salole EG

更新日期：1987-01-01 00:00:00

abstract：:An immunoassay based on fluorescence polarization detection (FPIA) has been recently adapted for dibekacin. This has been compared with a reference method (high-performance liquid chromatography), and two other methods used in clinical laboratories for monitoring this aminoglycoside, namely substrate-labelled fluoresc...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rollman B,Van der Auwera P,Tulkens PM

更新日期：1986-01-01 00:00:00

abstract：:A reversed-phase gradient elution system with methanol-triethylammonium phosphate buffer (83.3 mM, pH 6.0) as eluent on C(18)-bonded silica is described for the separation of 38 ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides in less than 33 min. The retention of the nucleotides can b...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Willis CL,Lim CK,Peters TJ

更新日期：1986-01-01 00:00:00

abstract：:The method for (1)H NMR determination of aspirin, phenacetin and caffeine (APC) mixtures has been improved by the use of 1,3,5-trioxane as an internal standard. The trioxane absorption occurs in a peak-free region of the spectrum and produces no interferences with any of the analytes. Compared to the original method w...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Eberhart ST,Hatzis A,Rothchild R

更新日期：1986-01-01 00:00:00

abstract：:The stability of a series of fluorescent isoindoles formed under analytical conditions following the reaction of o-phthalaldehyde (OPA) and ethanethiol (ET) with a series of primary amines is reported. Increasing the bulk and degree of substitution of the isoindole N-substituent resulted in substantial increases in is...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Stobaugh JF,Repta AJ,Sternson LA

更新日期：1986-01-01 00:00:00

abstract：:The 60 MHz (1)H NMR spectra of racemic 6,7-dichloro-5-methoxy-2-methyl-2-phenyl-1 indanone (1) have been studied with the achiral shift reagent, tris(6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octanedionato) europium (III) (2) and the chiral reagents, tris[3-(trifluoromethylhydroxymethylene)-d-camphorato] europium (II...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hatzis A,Rothchild R

更新日期：1986-01-01 00:00:00

abstract：:In this brief review a superficial introduction is given to the important area of biologically active carbohydrates. The complexity of carbohydrate chemistry is considered and the possible uses of carbohydrates as drugs and diagnostic agents are outlined. The analytical tools and methods that are especially useful for...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Bergström K

更新日期：1986-01-01 00:00:00

abstract：:It is shown that activity in the area of chemically modified electrodes continues at a high level. This topic is increasing in popularity with a large research field on the incorporation of electroactive groups into a variety of conductive films. These developments are also directed towards catalyzing bioselective and...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Patriarche GJ

更新日期：1986-01-01 00:00:00

abstract：:The alkaline hydrolysis of mitomycin C has been studied over a wide range of pH/H_(7-15). A stability-indicating high-performance liquid chromatographic (HPLC) method was used to separate the degradation products from the parent drug. The quantitative effects of temperature and buffers on the degradation of mitomycin ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Beijnen JH,den Hartigh J,Underberg WJ

更新日期：1985-01-01 00:00:00

abstract：:From the reaction between l-lysine and formaldehyde, Nepsilon-formyl-L-lysine was isolated by means of ion-exchange column chromatography. The identification of Nepsilon-formyl-L-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Tyihák E,Trézl L,Kolonits P

更新日期：1985-01-01 00:00:00

abstract：:A high-performance liquid chromatographic method for the determination of the theophylline solubilizer salicylamide-O-acetic acid has been developed in the range 0.5 to 10 microg/ml for human serum and 5 to 400 microg/ml for urine. Reversed-phase ion-pair chromatography was employed with tetrabutylammonium hydrogen su...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Schulz HU,Kraas E

更新日期：1985-01-01 00:00:00

abstract：:Microcolumn techniques involving pre-column concentration and post-column derivatization make good use of micro-scale high-performance liquid chromatography. The efficient use of microcolumns is illustrated by some applications to the analysis of amino acids, bile acids and catecholamines. The potential of new detecto...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ishii D,Goto M,Takeuchi T

更新日期：1984-01-01 00:00:00

abstract：:Flunixin is determined in equine plasma by liquid chromatography on LiChrosorb RP-18 with 70% methanol in phosphate buffer pH 3.1 as the eluent, with detection at 284 nm. Plasma is deproteinized with methanol and the supernatant is then injected directly into the system. With a short pre-column (5 x 3 mm i.d.), which ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Johansson IM,Schubert B

更新日期：1984-01-01 00:00:00

abstract：:The 60-MHz 1H NMR spectra of racemic mephenytoin, I, have been studied with the achiral shift reagent, tris(6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-3,5-octane-dionato) europium (III), II, and the chiral reagent, tris[3-(trifluoromethylhydroxy-methylene)-d-camphorato] europium (III), III. Moderate values of the enantiom...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Avolio J,Rothchild R

更新日期：1984-01-01 00:00:00

abstract：:The response of tumours to adriamycin, and the cardiotoxicity of the drug, may be related to its pharmacokinetics and plasma levels. Rapid and sensitive methods of adriamycin determination in plasma and urine samples are thus needed. A comparative study shows that high-performance liquid chromatography with fluorimetr...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rahmani R,Gil P,Martin M,Durand A,Barbet J,Cano JP

更新日期：1983-01-01 00:00:00

abstract：:A short review of the principles of atomic absorption spectrometry (AAS) - sources, atomizers, background correction, instrumentation, sample preparation, sensitivity, limit of detection, etc. - is given. The technique can be applied for impurity tests for trace metals as well as for assay of various commonly occurrin...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ali SL

更新日期：1983-01-01 00:00:00

abstract：:Determination of drugs in foods. In foods, drugs can come from different sources: animal therapeutics, zootechnical feed supplementation or human food additives. The possible consequences arising from the presence of drugs in food are considered. The quantitative determination of drugs in feeds or in human foods must ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Alary J

更新日期：1983-01-01 00:00:00